Invitrogen EVOS M5000 Fluorescence Imaging System
Specifications:
A fully integrated digital inverted microscope
Autofocus, Z-stack capability, single-click multichannel acquisition
4-color fluorescence (DAPI, GFP, Texas Red, Cy5), transmitted light, color imaging
4x, 10x, 20x objectives
Software for image acquisition, analysis, cell counting
Agilent Cytation C10 Confocal Fluorescence Microscope and Plate Reader
Specifications:
State of the art imaging technologies combined in one compact system
Automated spinning disk confocal microscopy
Wide field microscopy, multimode detection
20x, 40x, 60x H20 Olympus plan apochromate (APO) objective
CY5, DAPI, GFP, TRITC
Compatible with culture plates 6- to 1536-well, cell culture flasks and dishes, glass slides
Plate reading capability for fluorescence, UV-Vis absorbance and luminescence
Live cell imaging capability - temperature controller and gas controller for CO2, shaker
GEN5IPRIME image analysis and quantification software
Agilent Novocyte 2000R Flow Cytometer with Autosampler
Specifications:
The Novocyte 200R Flow cytometer has two lasers: Red and Blue, four fluorescence channels: FITC, Alexa488 (530/30), PE (572/28), PerCP (675/30), APC, Alexa647) (675/30)
The NovoSampler Pro enables the use of the following sample input formats: 12x75 mm, 5 mL tubes in a 24 tube rack, 24-well (standard) microwell plates, 48-well microwell plates, 96-well (flat-bottom, U-bottom, V-bottom) microwell plates. It does sample agitation by platform-based mixing
The Novocyte 200R Flow cytometer - NovoSampler Pro combo allows sample automation
The NovoExpress software enables operation, maintenance and troubleshooting of the flow cytometer. It enables data acquisition and analysis that is fully integrated into the flow cytometry system. It offers all the tools needed to collect and analyze data. It also enables data export in FCS3.0, FCS 3.1 and CSV formats for third-party software analysis. It provides the ability to acquire and analyze data simultaneously in conjunction with the NovoSampler Pro’s operation.
Vizgen Merscope Ultra
Specifications:
The MERSCOPE Ultra by Vizgen is a platform for advanced spatial transcriptomics measurements. It achieves sub-cellular imaging down to 100 nm, enabling detailed visualization of tissue architecture at the single-cell and sub-cellular levels.
The MERSCOPE Ultra enables extensive Multiplexing: The platform supports high-plex custom panel designs, enabling comprehensive analysis of complex tissue samples.
MERSCOPE Ultra enables comprehensive Tissue Coverage. It can process up to 3 cm² of tissue per day with single-molecule resolution across the entire tissue slice and provide a complete spatial context.
Invitrogen iBright FL 1500 Imaging System
Specifications:
Fluorescent western blots (RGB fluorescence and Near-IR fluorescence)
Chemiluminescent western blots
Protein gel imaging
DNA gel imaging
BioRad CFX OPUS 96 RT PCR System
Specifications:
96-well, 5-color plus FRET, network-connected real-time PCR detection system
Seahorse XFlex by Agilent
Seahorse XFlex by Agilent
Specifications:
The Seahorse XFlex is a flexible 24-well system that allows real-time metabolic analysis with improved sensitivity and consistency, using two-dimensional (2D) and three-dimensional (3D) study models.
It performs noninvasive, nondestructive measurements that do not require the addition of dyes, labels, or reporters.
Multiple parameters are reported from each assay well. The Seahorse simultaneously measures oxygen consumption rate (OCR) and extracellular acidification rate (ECAR) of living cells. Using these measurements, glycolytic proton efflux rate (PER) is calculated as an accurate measurement of glycolysis rates in real time. Quantitative ATP production rates can also be calculated in real time using well-validated algorithms to obtain mitoATP and glycoATP production rates, as well as total ATP production rate.
The Seahorse has a four-port injection system with an automated mixing feature for assessing immediate cellular, tissue organoid responses to substrates, inhibitors, and other compounds in real time.
Its intuitive, user-guided software supports easy assay protocol creation.
Liquid Nitrogen Storage
Azenta A220 Cryo Freezer
Specifications:
Provides secure -190oC liquid nitrogen vapor storage
44 slim vertical racks, 5 boxes per rack
Vial capacity: 22000
Currently mainly used for long-term liquid nitrogen storage
Accessed by LSCBE staff only
Locator6 Plus Cryogenic Storage
Specifications:
Provides secure Liquid Nitrogen storage within the facility
6 racks, 10 boxes per rack
Vial capacity: 6000
Can be accessed by LSCBE users
